About
Applying over 30 years of using flow cytometry in cancer and autoimmune research, new…
Articles by Dana
-
SoCal flow meeting
SoCal flow meeting
Don't forget to register for the SoCal flow meeting Apr. 25-26.
1 Comment
Activity
4K followers
-
Dana Buckman reposted thisDana Buckman reposted thisHeavy metals — cadmium, chromium, mercury, lead — are persistent, non-degradable contaminants of the food chain. EFSA's figures place dietary cadmium exposure in European adults at the boundary of the Tolerable Weekly Intake. The gut is where ingested metals become systemic burden. We assessed three single-strain lactobacilli — Lactiplantibacillus plantarum LP14, Lactobacillus crispatus LCR04, Lactobacillus acidophilus LA12 — along a multilevel ladder: static in vitro, dynamic SHIME, gut-ex-vivo at CAAD-UPO. Viable cell concentration was tracked compartment by compartment by flow cytometry in AFU; ICP-MS partitioned each metal between supernatant and cell pellet, the resolution required to distinguish sequestration from matrix artefact. Three findings emerged. In the colonic phase of the SHIME, LCR04 displaced ~45 percentage points of lead from supernatant into pellet relative to blank, 20–40 for cadmium and chromium with LCR04 and LP14, ~10 for mercury. Lead, refractory in static broth, became reachable the moment the strain was given a colonic environment. LA12, the strongest static performer, failed to proliferate in the colon and removed no metal. Sequestration tracked metabolic activity, not biomass: only strains expanding by ~2 log AFU — LP14 and LCR04 — achieved meaningful removal. In the gut-ex-vivo on mouse small intestine, the metal mixture compromised barrier integrity: paracellular permeability rose, claudins and occludin dysregulated, TNF-α and IFN-γ elevated, IL-10 suppressed. Pre-incubation with any of the three cell-free supernatants restored the phenotype: permeability to baseline, transcripts in range, cytokines toward homeostasis. Sadly, heavy-metal contamination is a chronic-exposure problem the regulatory machinery cannot eliminate at source. What our data support is a deliberately narrow proposition. A properly selected single-strain probiotic, validated along a transit-realistic ladder, could operate as a gut-level adjunct to existing food-safety measures. Not a substitute for source control. Not a clinical treatment. An adjunct — a further layer of protection at the interface where ingested metals become systemic burden. This paper, just out in Frontiers in Microbiology, is my PhD thesis — Cycle XXXVI of the Food, Health and Longevity programme at Università del Piemonte Orientale. Open access. DOI in first comment. #probiotics #flowcytometry #shime #publichealth #heavymetals #foodsafety #microbiome
-
Dana Buckman reposted thisDana Buckman reposted thisWe are proud to share that our R&D team presented 𝘁𝘄𝗼 𝘀𝗰𝗶𝗲𝗻𝘁𝗶𝗳𝗶𝗰 𝗽𝗼𝘀𝘁𝗲𝗿𝘀 at the 14𝘵𝘩 𝘔𝘪𝘤𝘳𝘰𝘣𝘪𝘰𝘮𝘦 𝘙&𝘋 𝘢𝘯𝘥 𝘉𝘶𝘴𝘪𝘯𝘦𝘴𝘴 𝘊𝘰𝘭𝘭𝘢𝘣𝘰𝘳𝘢𝘵𝘪𝘰𝘯 𝘍𝘰𝘳𝘶𝘮 in Rotterdam, highlighting our latest research on Skinbac solutions for personal care applications. The studies explored two key areas of microbiome-friendly personal care: 🔬 “An Inside-Out Approach to Scalp and Hair Health: 𝘐𝘯 𝘝𝘪𝘵𝘳𝘰 and 𝘐𝘯 𝘝𝘪𝘷𝘰 Evidence” - Annalisa Visciglia, Giovanni Deusebio, Angela Amoruso, Marco Pane. This work investigated a combined topical and oral strategy designed to support 𝗵𝗲𝗮𝗹𝘁𝗵𝗶𝗲𝗿 𝘀𝗰𝗮𝗹𝗽 and 𝗵𝗮𝗶𝗿 𝗵𝗲𝗮𝗹𝘁𝗵. 𝘐𝘯 𝘝𝘪𝘵𝘳𝘰, 𝗦𝗸𝗶𝗻𝗯𝗮𝗰 𝗦𝗕𝟬𝟱 supported dermal papilla fibroblast activity and helped modulate 5α-reductase type 1 in testosterone-stimulated keratinocytes. 𝘐𝘯 𝘝𝘪𝘷𝘰, the oral supplement and topical serum combination delivered visible improvements in key scalp and hair parameters after 90 days, including scalp hydration, hair brightness, hair density, hair diameter and the anagen/telogen ratio. 🔬 “Sweat Less, Smell Better: 𝘐𝘯 𝘝𝘪𝘵𝘳𝘰 and 𝘐𝘯 𝘝𝘪𝘷𝘰 Evaluation of Heat-Treated Lacticaseibacillus rhamnosus Skinbac SB06, a Postbiotic Antiperspirant-Deodorant Active” - Giovanni Deusebio, Annalisa Visciglia, Angela Amoruso, Marco Pane. This work investigated 𝗦𝗸𝗶𝗻𝗯𝗮𝗰 𝗦𝗕𝟬𝟲 as a postbiotic active for underarm care. 𝘐𝘯 𝘝𝘪𝘵𝘳𝘰, Skinbac SB06 showed a selective microbiome-modulating profile, preserving the skin commensal 𝘚. 𝘦𝘱𝘪𝘥𝘦𝘳𝘮𝘪𝘥𝘪𝘴 while reducing 𝘊. 𝘴𝘵𝘳𝘪𝘢𝘵𝘶𝘮 and 𝘚. 𝘩𝘰𝘮𝘪𝘯𝘪𝘴, two microorganisms associated with body odor formation. 𝘐𝘯 𝘝𝘪𝘷𝘰, a 4-day treatment cycle reduced sweat by up to 22% and significantly lowered odor intensity for up to 48 hours, 𝘄𝗶𝘁𝗵𝗼𝘂𝘁 𝘁𝗵𝗲 𝘂𝘀𝗲 𝗼𝗳 𝗮𝗹𝘂𝗺𝗶𝗻𝘂𝗺 𝘀𝗮𝗹𝘁𝘀. These findings reinforce our commitment to science-driven innovation and to the development of effective, microbiome-friendly personal care solutions. 📌 Read more: https://lnkd.in/dyBZq3sS #Microbiome #PersonalCare #Postbiotics #SkinHealth #HairCare #Innovation #ResearchAndDevelopment #Skinbac #Deocare
-
Dana Buckman shared thisThis is fantastic to see. Solid clinical validation is exactly what the biotic space needs right now to differentiate and drive credibility. Kudos to the Probiotical team for investing in the science that moves the whole industry forward. 🔬✨Dana Buckman shared this“Clinically studied” on a supplement label can mean almost anything — from a single pilot in healthy volunteers to a multi-year programme across independent research groups. The difference is not semantic. It is the distance between a data point and a body of evidence. Over the past ten years, we have built one of the most comprehensive clinical programmes in psychobiotics around a single four-strain formula: L. fermentum LF16, L. rhamnosus LR06, L. plantarum LP01, B. longum 04. Four billion live cells per day. Eight peer-reviewed publications. Four independent trials: Italy, US, Australia. Marotta et al. (2019) established proof of concept in 38 healthy adults: depression scores down 44%, anger down 37%, sleep quality improved 29% — all persisting three weeks after washout. It was also the first study with published stability data reported in AFU and enumerated by Flow Cytometry. Calgaro et al. (2021) confirmed strain recovery and three baseline bacteriotypes predicting response. Maltodextrin placebo showed a bifidogenic effect — sharpening every subsequent study. Walden et al. (2023) scaled to 70 subjects in the largest dedicated psychobiotic RCT. Anxiety and rumination decreased significantly versus placebo. Plasma serotonin rose at six and nine weeks — no change in placebo. Kerksick et al. (2024), on the same cohort, confirmed significant improvements in sleep quality, latency, and disturbance (PSQI). Di Cesare et al. (2023) added the metabolomic layer: 600 MHz NMR, 840 urine samples, 80% classification accuracy. The discriminating metabolites — hippurate, hydroxyphenylacetate, formate, amino acid shifts — all bacteria-derived. Moschonis et al. (2026): a clinical population with subthreshold depression, DSM-5 diagnosed (conducted during COVID!). Questionnaires replaced by blood and saliva. Fasting glucose decreased with a significant treatment-by-time interaction. hs-CRP decreased. Glutathione — the body’s primary endogenous antioxidant — increased at both six and twelve weeks. Cortisol decreased significantly. Dacaya et al. (2025) documented the SSRI-like serotonin regulation — the biphasic rise-then-fall mirroring antidepressant pharmacodynamics. The follow-up (Moschonis et al., 2026) expanded the picture. LBP and sCD14 — markers of endotoxin translocation — decreased significantly, indicating barrier strengthening. GIP, an enteroendocrine hormone, also decreased, suggesting metabolic benefits beyond mood. We named this findings BIFIZEN! Five layers: microbiome, metabolome, serotonin, inflammation, clinical outcomes. The most significant findings — bacteriotype response, biphasic serotonin, barrier strengthening — were not in the original hypotheses. Eight publications. Three countries. Ten years. That is what “clinically studied” looks like when you mean it :) Full biblio in the first comment. #psychobiotics #probiotics #gutbrainaxis #clinicaltrials #microbiome #stress #anxiety #sleep #BIFIZEN
-
Dana Buckman shared thisJoin me in beautiful Vancouver for the upcoming IPA + Probiota Americas meeting! The momentum in our industry right now is incredible. I’m excited to look at where this fast-moving space is headed—let's connect while we're there!Dana Buckman shared thisMost viability measurements tell you what already happened. What if they could help guide manufacturing decisions in real time instead? At Probiota Events 2026 in Vancouver, our CEO at BioForm Solutions, Dana Buckman, and Dr. Rishi R. Trivedi, PhD from Biose Industrie will be sharing a collaborative poster focused on applying flow cytometry as a real-time process tool for next-generation probiotic manufacturing. For highly sensitive anaerobic organisms like Akkermansia muciniphila, traditional plating methods can create long feedback loops that are difficult to use during active manufacturing. This work explores how faster physiological insights can support better decision-making across fermentation, downstream processing, formulation, and stability monitoring. As the NGP space continues to evolve, the industry needs analytical approaches that move beyond simple enumeration toward a deeper understanding of microbial health and product robustness throughout the manufacturing lifecycle. If you’ll be at Probiota, Dana would love to connect and continue the discussion around the future of real-time viability monitoring in microbiome therapeutics. #NGP #microbialhealth #probiotics #microbiome https://lnkd.in/gYPMsKP4
-
Dana Buckman reposted thisDana Buckman reposted thisWe are looking for new colleagues. So, if you love sciences and probiotics- check out this opportunity at Probi!
-
Dana Buckman reposted thisDana Buckman reposted thisThe probiotic industry has unlocked incredible potential—but performance in real world products is what matters most. At Probiota Americas, the IFF Health Sciences team is excited to contribute across multiple touchpoints — hosting a breakfast briefing, leading a roundtable discussion, and joining an expert panel — all focused on turning microbiome science into impact. We’re looking forward to meaningful conversations that move the category forward. #ProbiotaAmericas #ThoughtLeadership #MicrobiomeInnovation
-
Dana Buckman reposted thisDana Buckman reposted thisWe're #hiring a new Gummy R&D Technician in Anaheim, California. Apply today or share this post with your network.
-
Dana Buckman reposted thisDana Buckman reposted thisWe don’t have a probiotic demand problem. We have a validation problem. The team here at BioForm Solutions came across this recent piece on why probiotics often don’t work and it reinforces something many of us in the space have been seeing firsthand. From strains that aren’t actually effective, to viability issues, to the simple reality that some microbiomes won’t even accept new bacteria - the variability is real. And yet… the category continues to grow. That’s the tension right now. The next phase of this industry won’t be driven by more SKUs or better marketing. It’s going to be driven by precision, measurement, and proof - understanding not just what’s in the product, but what’s alive, functional, and actually doing something meaningful. Because if we can’t consistently answer that, the rest doesn’t matter. Curious how others are thinking about this - are we finally at the point where validation becomes the differentiator? https://lnkd.in/grqf4PJF
-
Dana Buckman liked thisSuch an exciting time to be in this field. Did you know that microbes amplify our genome! The microbial cells living in and on our bodies collectively contain far more genes than our own human genome, giving our microbiome an enormous metabolic capability! Humans didn’t evolve on their own. Thank your microbes the next time you take a bite!Dana Buckman liked this🌍 Today is World Digestive Health Day! Digestive health is more than digestion, it influences immunity, metabolism, and overall well-being. Probiotics, prebiotics, and postbiotics each play important roles in supporting a healthy gut microbiome and digestive health. The International Probiotics Association (IPA) celebrates the healthcare professionals, scientists, regulators, and industry leaders working to advance evidence-based microbiome science! #WorldDigestiveHealthDay #DigestiveHealth #GutHealth #Microbiome #Probiotics #Prebiotics #Postbiotics #IPA #NutritionScience
-
Dana Buckman liked thisOlawale thank you for sharing this Gardentastic post. These tips are very helpful for a cranmazing gardenDana Buckman liked this💡The Seeds of Today are the Plants of Tomorrow!🤯 Planting embodies patience, nurturing each seed with faith, knowing growth takes time and care. Watching plants grow in a small space can be fun, #teamwork and rewarding #experience, whether you're a seasoned gardener or just getting started. These are simple and useful tricks & methods to grow plants in your own #home or anywhere with the #children & #family. Every small action today plants something that could bloom into your #future. Write-up: Olawale Kolawole #education #science #innovation #environment #business #motivation #ai #inspiration #bestadvice #leadership #cfbr #economy #mindfulness #creativity #hr #healthcare #sustainability #agriculture #construction #art #manufacturing #finance #warehouses #management #students #strategy #whatinspiresme #technology #future #humanresources #advertisingandmarketing #nature
-
Dana Buckman liked thisDana Buckman liked thisProbi, part of Symrise AG, has achieved a key milestone with the first-ever clinical study of LP299V® in Japanese adults. The randomized, placebo-controlled study showed significant improvements in bowel habits, reinforcing LP299V’s ability to deliver clinically meaningful outcomes in the Asian cohort. Backed by a well-established mechanism related to its ability to survive gastric transit, adhere to the gut lining, and support growth of butyrate-producing bacteria, LP299V continues to set the standard for science-based probiotics. As the demand for credible, evidence-based health solutions grows, Probi remains focused on advancing locally validated, science-backed probiotics that create real value for partners and consumers. Learn more here: https://lnkd.in/dR29rXyc #probiotics #guthealth #japan #backedbyscienceProbi and Nomura Dairy achieve first clinical validation of LP299V® in Japanese adults, advancing science-led probiotic innovation in AsiaProbi and Nomura Dairy achieve first clinical validation of LP299V® in Japanese adults, advancing science-led probiotic innovation in Asia
-
Dana Buckman liked thisDana Buckman liked thisI can't wait to be speaking at the #Probiota Americas 2026. Join me in Vancouver on June 8 - 10, for the leading microbiome industry event in North America! Join me and book your spot: https://lnkd.in/eYcdGtN6 #NutraIngredients
-
Dana Buckman liked thisDana Buckman liked thisEvery time I see a probiotic label that lists "𝘓𝘢𝘤𝘵𝘪𝘱𝘭𝘢𝘯𝘵𝘪𝘣𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝘱𝘭𝘢𝘯𝘵𝘢𝘳𝘶𝘮" and stops there… (Yes, the strain is the entire point. The species is just the postcode.) In probiotics, the strain is the grape, the vintage and the estate. A wine list that just says "red" is not a wine list. It is a guess. The species level on a probiotic label is the same guess in a lab coat. Footnote: Two strains of 𝘓𝘢𝘤𝘵𝘪𝘱𝘭𝘢𝘯𝘵𝘪𝘣𝘢𝘤𝘪𝘭𝘭𝘶𝘴 𝘱𝘭𝘢𝘯𝘵𝘢𝘳𝘶𝘮 can sit in completely different evidence bases, with completely different GI, immune or metabolic dossiers behind them. The DSM, CECT, ATCC or NCIMB code in tiny grey font on the back of the box is doing all the scientific work. The big number on the front of the box, the lonely species name, the green leaf icon are doing none of it. If the strain code is missing, the trial is missing.
-
Dana Buckman liked thisDana Buckman liked thisPreparing for #Probiota Americas required a few extra helping hands this week. 🙌 400 conference giveaways packed. Countless stickers applied. One dining room temporarily converted into a packing operation. 📦 No one in the family was safe from being recruited. The contents remain classified until Vancouver. 😉 Looking forward to great conversations around #microbiome science, strain specificity, and why complex systems aren't solved by chance. See you at Probiota Americas!!
-
Dana Buckman liked thisDana Buckman liked thisIn just over a week, I’ll be in Vancouver for #ProbiotaAmericas2026 and cannot wait to meet with industry leaders and partners across probiotics, microbiome, and wellness to discuss innovation, new opportunities, and where our market is heading next 🚀 Message me or comment below if you’ll be attending and are interested in connecting — I would love to schedule time while we’re there 🤝 See everyone Monday, June 8th! #Probiota #Microbiome #Wellness #Nutraceuticals #NextGenerationBiotics #TeamworkDreamwork
Experience & Education
-
BioForm Solutions
View Dana’s full experience
See their title, tenure and more.
or
Licenses & Certifications
-
International Cytometry Certification Examination (ICCE)
-
Issued Expires -
Basic cytometry service
CytoServ
-
Clinical Flow Cytometry
AACC
Publications
-
[18F]FLT-PET imaging does not always “light up” proliferating tumor cells
Manuscript in Submission
Other authors -
-
A novel SND1-BRAF fusion confers resistance to c-Met inhibitor PF-04217903 in GTL16 cells though MAPK activation
Manuscript submitted to Cancer Research
Other authors -
-
Application of flow cytometry in drug discovery
European Pharmaceutical
-
Dual Functional Monoclonal Antibody PF-04605412 Targets Integrin alpha5 beta1 and Elicits Potent Antibody-Dependent Cellular Cytotoxicity
Cancer Research
Other authors -
-
PF-00477736 mediates checkpoint kinase 1 signaling pathway and potentiates docetaxel-induced efficacy in xenografts
Clinical Cancer Research
Other authors -
-
CD10 is a key enzyme involved in the activation of tumor-activated peptide prodrug CPI-0004Na and novel analogues: Implications for the design of novel peptide prodrugs for the therapy of CD10+ tumors
Cancer Research
Other authors -
-
Binding to CD20 by anti-B1 antibody or F(ab’)(2) is sufficient for induction of apoptosis in B-cell lines
Cancer Immunol Immunother
Other authors -
Courses
-
Data Mining
UCSD
-
Effective Strategies for Teaching Adults
UCSD
-
Industrial Biotechnology (IB)
BioCom
-
Life Science Immersion Program (LSIP)
BioCom
-
Mandarin for travellers
UCSD
Recommendations received
10 people have recommended Dana
Join now to viewOther similar profiles
Explore more posts
Explore top content on LinkedIn
Find curated posts and insights for relevant topics all in one place.
View top content