Greater São Paulo Area
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I have a Bachelor's degree in Computer Engineering, an MBA in Data Engineering from FIAP,…

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Publications

  • Chromatin landscape distinguishes the genomic loci of hundreds of androgen-receptor-associated lincRNAs from the loci of non-associated lincRNAs

    Frontiers In Genetics

    Cell signaling events triggered by androgen hormone in prostate cells is dependent on activation of the androgen receptor (AR) transcription factor. Androgen hormone binding to AR promotes its displacement from the cytoplasm to the nucleus and AR binding to DNA motifs, thus inducing activatory and inhibitory transcriptional programs through a complex regulatory mechanism not yet fully understood. In this work, we performed RNA-seq deep-sequencing of LNCaP prostate cancer cells and found over…

    Cell signaling events triggered by androgen hormone in prostate cells is dependent on activation of the androgen receptor (AR) transcription factor. Androgen hormone binding to AR promotes its displacement from the cytoplasm to the nucleus and AR binding to DNA motifs, thus inducing activatory and inhibitory transcriptional programs through a complex regulatory mechanism not yet fully understood. In this work, we performed RNA-seq deep-sequencing of LNCaP prostate cancer cells and found over 7000 expressed long intergenic non-coding RNAs (lincRNAs), of which ~4000 are novel lincRNAs, and 258 lincRNAs have their expression activated by androgen. Immunoprecipitation of AR, followed by large-scale sequencing of co-immunoprecipitated RNAs (RIP-Seq) has identified in the LNCaP cell line a total of 619 lincRNAs that were significantly enriched (FDR < 10%, DESeq2) in the anti-Androgen Receptor (antiAR) fraction in relation to the control fraction (non-specific IgG), and we named them Androgen-Receptor-Associated lincRNAs (ARA-lincRNAs). A genome-wide analysis showed that protein-coding gene neighbors to ARA-lincRNAs had a significantly higher androgen-induced change in expression than protein-coding genes neighboring lincRNAs not associated to AR. To find relevant epigenetic signatures enriched at the ARA-lincRNAs’ transcription start sites (TSSs) we used a machine learning approach and identified that the ARA-lincRNA genomic loci in LNCaP cells are significantly enriched with epigenetic marks that are characteristic of in cis enhancer RNA regulators, and that the H3K27ac mark of active enhancers is conspicuously enriched at the TSS of ARA-lincRNAs adjacent to androgen-activated protein-coding genes. In addition, LNCaP topologically associating domains (TADs) that comprise chromatin regions with ARA-lincRNAs exhibit transcription factor contents, epigenetic marks and gene transcriptional activities that are significantly different from TADs not containing ARA-lincRNAs.

    Other authors
    • Lucas F daSilva
    • Felipe C. Beckedorff
    • Ana C. Ayupe
    • Murilo S. Amaral
    • Alexandre Videira
    • Eduardo M Reis
    • Joao Carlos Setubal
    • Sergio Verjovski-Almeida
    See publication
  • Blog pessoal de Tecnologia

    vmesel

    Blog sobre os diversos assuntos de tecnologia que ando tratando.

    See publication

Courses

  • Introduction to Computer Vision

    CS 4476/6476

Projects

Honors & Awards

  • Artigo escolhido para a Revista InCiência

    Colégio Dante Alighieri

  • Primeiro lugar no VIII Simpósio de Ciência do Colégio Dante Alighieri

    Colégio Dante Alighieri

Languages

  • Inglês

    Native or bilingual proficiency

  • Espanhol

    Limited working proficiency

  • Hebraico

    Elementary proficiency

Organizations

  • GruPy

    Coordenador e Coorganizador

    - Present

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