JMSACL (Journal of Mass Spectrometry & Advances in the Clinical Lab)

Source: (Methods in molecular biology (Clifton, N.J.)) HPLC-HRMS method for extracting, purifying, and quantifying cytokinins (CKs) from mammalian tissues is described. CKs, plant hormones found in low abundance in animals, require precise detection. The protocol involves sample homogenization, methanol extraction, solid-phase extraction to remove impurities, and analysis via HPLC and Orbitrap mass spectrometry, achieving high recovery and sensitivity for CK studies.

Source: (Rapid communications in mass spectrometry : RCM) Mass spectrometry distinguishes sickle cell trait (SCT) from sickle cell disease (SCD) using hemoglobin analysis. An optimal β<S>/β ratio cut-off of 0.71 shows high sensitivity (90.3%) and specificity (98.4%). Additional globin ratios refine SCD subclassification. This rapid, cost-effective method may reduce reliance on molecular testing.

Source: (Journal of mass spectrometry : JMS) Developed LC-HR-MS method with multiple fragmentation modes enhances identification of hemoglobin variants in clinical diagnosis, improving resolution and accuracy. Utilizes C4 column and modes (HCD, EThcD) for high sequence coverage. Analyzed clinical samples show efficacy in distinguishing isomeric variants, reducing reliance on costly genetic tests.

Source: (Clinica chimica acta; international journal of clinical chemistry) Study establishes age-specific reference intervals for ethanolamine plasmalogen species in red blood cells, showing significant age-dependent changes. Plasmalogens, crucial for membrane integrity, are lower in neonates and vary with age. Eighteen species were quantified using UPLC-MS/MS in 441 healthy individuals. Results support separate pediatric and adult reference intervals, aiding plasmalogen testing in clinical labs.

Source: (Journal of visualized experiments : JoVE) Plasma proteomics via mass spectrometry enables biomarker discovery for systemic diseases. Advances in high-throughput technologies improve protein identification and quantification, addressing past limitations like poor reproducibility. The study presents a scalable workflow integrating immunodepletion, filtration, and LC-MS/MS profiling, enhancing proteome depth and reducing variability. This method supports accurate differential protein expression analysis, aiding biomarker-driven diagnostics and personalized medicine.

Source: (Journal of clinical microbiology) MALDI-TOF MS combined with machine learning enhances detection of KPC-producing Klebsiella pneumoniae, addressing the global challenge of carbapenem resistance. This study utilized 435 clinical isolates, achieving over 95% specificity and 72% sensitivity, surpassing traditional methods. An annotated MALDI-TOF MS spectrum was developed for peak identification related to antimicrobial resistance, offering a resource for further analysis.

Source: (ACS omega) Mass spectrometry (MS)-based metabolomics and machine learning (ML) effectively diagnose Lyme neuroborreliosis (LNB), distinguishing between acute LNB, treated LNB, and non-LNB controls. Analyzed serum samples showed strong discriminatory performance, with ML models achieving high accuracy, especially between treated LNB and non-LNB groups. ROC analyses confirmed high sensitivity and specificity, demonstrating the potential of metabolomic profiling and ML for LNB diagnosis despite limited variance in unsupervised models.

Source: (Analytical chemistry) Vinyltrimethylsilane chemical ionization tandem mass spectrometry effectively distinguishes methoxyphenylpiperazine isomers, generating unique fragment ions for each isomer. This method, supported by density functional theory, provides robust isomer identification crucial for forensic and pharmaceutical applications.

Source: (Microbiology spectrum) Study presents high-resolution proteotyping using MALDI-TOF MS and nano-LC-MS/MS to differentiate clinically relevant pathogens Haemophilus influenzae and Haemophilus aegyptius. Identified 31 unique protein biomarkers for accurate species-level classification, addressing limitations of traditional methods. This approach enhances diagnostic accuracy and supports better treatment decisions.

Source: (Journal of separation science) Galloylated B-type procyanidins from Rumex obtusifolius L. were identified and structurally elucidated using enzymatic digestion, phloroglucinolysis, and high-resolution mass spectrometry. Five dimeric procyanidins were analyzed, revealing their configurations and galloylation positions. This study presents the first report of these compounds in R. obtusifolius.