Server

cmd2web is a simple framework for enabling a web interface to command line programs. From this interface, data and methods can be easily accessed through either a dynamic website or a programmatic interface. This package includes a server and client javascript and python interfaces.

Server

To stand up a server, you define a mapping between command line parameters and URL query parameters in a JSON config file. Each command line instance is referred to as a service, and a server can host multiple services.

In this example, we will consider a server with just one service to the tabix program (https://github.com/samtools/htslib) and the Repeat Masker track from UCSC genome browser (based on http://hgdownload.soe.ucsc.edu/goldenPath/hg19/database/rmsk.txt.gz )

Install Tools

Installation Steps: Tabix and bgzip

Download latest tabix jar from the link:https://sourceforge.net/projects/samtools/files/tabix/ 
tar -xjf tabix-0.2.6.tar.bz2
cd tabix-0.2.6/
make
Make install

Bedtools: Run the following lines in the terminal to install bedtools:

Ubuntu
apt-get install bedtools

Mac
brew tap homebrew/science
brew install bedtools

After downloading just extract the .gz file and use head command to see content of the file:

Extract the bgzip file:

bgzip -d rmsk.txt.gz
Head rmsk.txt
mv  rmsk.txt rmsk.bed

For tabix to work start position and end position should be sorted: Here column 6 is the chromosome and column 7,8 is the starting and the ending position

sort -k6,6 -k7,7n -k8,8n rmsk.bed >> sortedrmsk.bed

Once the file is created, use bgzip to compress the .bed file:

Bgzip sortedrmsk.bed

It will create sortedrmsk.bed.gz file which can be used with the tabix. To create index file(.tbi) using tabix use the following command:

tabix -p bed sortedrmsk.bed.gz -s6 -b7 -e8

Parameters in the command:

-p bed - Tells about the type of input file
Sortedrmsk.bed.gz - Input file
-s6 - Tells about the column of the sequence (In this case 6th column)
-b7 - Tells about the column having starting position of sequence ( In this case 7th column)
-e8 - Tells about the column having ending position of sequence ( In this case 8th column)

To test from terminal, use the following command:

tabix sortedextrarmsk.bed.gz -s6 -b7 -e8 chr1:10000-20000

Currently the application assumes that the columns in data file starts with the sequence. To convert the downloaded file to that, run the following commands:

Extract the bgzip file:
bgzip -d rmsk.txt.gz

Put columns in new file

cut -f6,7,8,9,10,11,12,13,14,15,16,17 rmsk.txt >> rmsk.bed

Sort the file

Sort -k1,1 -k2,2n -k3,3n rmsk.bed >> sortedrmsk.bed

Alternatively bedtools can be installed to sort the file:

Run the following lines in the terminal to install bedtools:

Ubuntu
apt-get install bedtools

Mac
brew tap homebrew/science
brew install bedtools

Once it is installed, use the following command to sort the input file

sortBed -i rmsk.bed

Use bgzip to compress the file:

Bgzip -c rmsk.bed >> rmsk.bed.gz

Use tabix to generate .tbi file

Tabix -p bed rmsk.bed.gz

Use tabix to filter by chromosome:

Tabix rmsk.bed.gz chr1:10000-20000

From the tabix manual:

Tabix indexes a TAB-delimited genome position file in.tab.bgz and creates an
index file ( in.tab.bgz.tbi or in.tab.bgz.csi ) when region is absent from the
command-line. The input data file must be position sorted and compressed by
bgzip which has a gzip(1) like interface.

After indexing, tabix is able to quickly retrieve data lines overlapping
regions specified in the format "chr:beginPos-endPos". (Coordinates specified
in this region format are 1-based and inclusive.)

To use tabix on the command line, you pass the file name and the region of interest and all intervals in the file that overlap that region are returned.

$ tabix rmsk.bed.gz chr1:10000-20000
chr1    10000   10468   (CCCTAA)n   Simple_repeat   Simple_repeat
chr1    10468   11447   TAR1    Satellite   telo
chr1    11503   11675   L1MC    LINE    L1
chr1    11677   11780   MER5B   DNA hAT-Charlie
chr1    15264   15355   MIR3    SINE    MIR
chr1    16712   16749   (TGG)n  Simple_repeat   Simple_repeat
chr1    18906   19048   L2a LINE    L2
chr1    19947   20405   L3  LINE    CR1

The URL for this service looks very similar to the command line invocation:

$ curl "http://127.0.0.1:8080/?service=rmsk&chromosome=chr1&start=10000&end=20000"
{ "success": 1,
  "result": [
    [ "chr1", "10000", "10468", "(CCCTAA)n", "Simple_repeat", "Simple_repeat" ],
    [ "chr1", "10468", "11447", "TAR1", "Satellite", "telo" ],
    [ "chr1", "11503", "11675", "L1MC", "LINE", "L1" ],
    [ "chr1", "11677", "11780", "MER5B", "DNA", "hAT-Charlie" ],
    [ "chr1", "15264", "15355", "MIR3", "SINE", "MIR" ],
    [ "chr1", "16712", "16749", "(TGG)n", "Simple_repeat", "Simple_repeat" ],
    [ "chr1", "18906", "19048", "L2a", "LINE", "L2" ],
    [ "chr1", "19947", "20405", "L3", "LINE", "CR1" ]
  ]
}

Server config

Config file has been updated from json to yaml file. A skeleton of the config is:

-
        name : 
        arguments : 
            -
                name : 
                fixed : 
                type : 
                value : 
        command :
            - 
        output :
            type : 
            sep :

Each element in the config is described below.

name

Since a server can host many services, the first step is to define the service name. Requests will use the service URL query attribute to the name of the service. The server will return an exception for any requests that do not have service specified:

$ curl "http://127.0.0.1:8080/?chromosome=chr1&start=10000&end=20000"
{"exception": "No service specified", "success": 0}

Here the name is rmsk:

"name" : "rmsk"

arguments

This command has five arguments:

path to the executable (tabix)
file of interest (rmsk.bed.gz)
chromosome (chr1)
start (10000)
end (20000)

Each of these attributes is specified in a arguments array. Attributes can be fixed ("fixed" : "true") or variable ("fixed" : "false)". The value of the variable attributes will be defined in a users' query, and the fixed attributes are defined by the value field. Each attribute has a type that is checked by the server before executing any commands. Currently string, integer, and float are supported.

The arguments array for this service in raw yaml is:

arguments : 
    -
        name : file
        fixed : 'true'
        type : string
        value : /data/rmsk.bed.gz
    -
        name : chromosome
        fixed : 'false'
        type : string
    -
        name : start
        fixed : 'false'
        type : integer
    -
        name : end
        fixed : 'false'
        type : integer

When a user requests this service, the server fills a variable table with both fixed and variable attributes. The server will return an exception for requests that do not provide precisely the set of variable arguments.

$ curl "http://127.0.0.1:8080/?service=rmsk&chromosome=chr1&start=10000"
{"exception": "Argument mismatch", "success": 0}

The server will also return an exception for requests with type mismatches.

$ curl "http://127.0.0.1:8080/?service=rmsk&chromosome=chr1&start=10000&end=not_int"
{"exception": "Type mismatch for argument end. Expected integer", "success": 0}

command

After the variable table is filled, the server will construct the command by replaced variables names with value in the variable table by the same name. Note that variables in the command start with '$', but the names of the attributes do not. To improve security, it is highly recommended to split the command into individual strings.

"command":
[
    "/usr/bin/tabix",
    "$file",
    "$chromosome:$start-$end"
],

The constructed command is then executed locally on the server. While it is beyond the scope of this example, we highly recommend running commands through a virtual machine such as docker.

The server will return an exception for any command that does not return with a zero exit code. Commands also have a maximum runtime that is specified by the --timeout option when starting the server. The server will return an exception if a command exceeds this limit.

output

The last step is to define the output type. Current text_stream and file are supported.

"output" :
{
    "type" : "text_stream",
    "sep" : "\t"
}

For text_stream output, the server returns a JSON array or arrays. Output is split by line then by the value given in the sep field.

staring the server

python server.py --config tabix_config.yaml

usage: server.py [-h] --config CONFIG [--port PORT] [--host HOST]
                 [--timeout TIMEOUT]

command to web server.

optional arguments:
  -h, --help         show this help message and exit
  --config CONFIG    Configuration file.
  --port PORT        Port to run on (default 8080)
  --host HOST        Server hos (default 127.0.0.1)
  --timeout TIMEOUT  Max runtime (sec) for a command (default 10)

In addition to serving requests, the server also advertises the services it supports along with input and output requirements. This information allows for the development of more general clients interfaces and can be accessed at the \info endpoint.

$ curl "http://127.0.0.1:8080/info"
{
    "rmsk": {
	"name": "rmsk",
	"output": { "type": "text_stream" },
        "inputs": [ 
          { "name": "chromosome", "type": "string" },
	  { "name": "start", "type": "integer" },
	  { "name": "end", "type": "integer" }
	]
      }
}

Client

Any method that can make web request can be a client. For example, curl works great.

$ curl "http://127.0.0.1:8080/?service=rmsk&chromosome=chr1&start=10000&end=20000"
{ "success": 1,
  "result": [
    [ "chr1", "10000", "10468", "(CCCTAA)n", "Simple_repeat", "Simple_repeat" ],
    [ "chr1", "10468", "11447", "TAR1", "Satellite", "telo" ],
    [ "chr1", "11503", "11675", "L1MC", "LINE", "L1" ],
    [ "chr1", "11677", "11780", "MER5B", "DNA", "hAT-Charlie" ],
    [ "chr1", "15264", "15355", "MIR3", "SINE", "MIR" ],
    [ "chr1", "16712", "16749", "(TGG)n", "Simple_repeat", "Simple_repeat" ],
    [ "chr1", "18906", "19048", "L2a", "LINE", "L2" ],
    [ "chr1", "19947", "20405", "L3", "LINE", "CR1" ]
  ]
}

This package also provides a python API that is tightly coupled with the server. For example, exceptions returned by the server are then raised as an exception by the API. The client will also perform local type checking on all service requests before submitting them to the server.

The client has two steps

connecting to the server with cmd2web.Client.connect(host, port)
running a query with run(service_name, **kwargs) where the kwargs map to the services variable arguments

To use the service defined above:

import cmd2web
s = cmd2web.Client.connect('127.0.0.1','8080')
R =  R = s.run('rmsk', chromosome="chr1", start=10000, end=20000)
for r in R:
    print('\t'.join(r))

Installation

The easiest way to install cmd2web is with virtualenv:

virtualenv -p /usr/local/bin/python3.6 cmd2web_env
source cmd2web_env/bin/activate
pip install flask requests numpy Cython cyvcf2 pyyaml pyopenssl flask-cors mod_wsgi werkzeug pandas

Apache Setup

To Install Apache:-

sudo apt-get update
sudo apt-get install apache2

To Add new site we need to add new configuration for our website in /etc/apache/sites-available:

sudo nano /etc/apache/sites-available/myweb.conf

<VirtualHost *:80>
     # Add machine's IP address (use ifconfig command)
     ServerName localhost
     # Give an alias to to start your website url with
     # Using cmd2web, it means in URL you have to write localhost to access the application. The path after that is path to your wsgi file. Change it accordingly.
     WSGIScriptAlias / somepath/flaskapp.wsgi
     # Mention the directory which should be accessible by your application. This will be directory which has all the files.
     <Directory /home/rohit/Documents/Academics/sem4/IndependentStudy/forkedrepo/cmd2web/>
     # set permissions as per apache2.conf file
            Options FollowSymLinks
            AllowOverride None
            Require all granted
     </Directory>
     ErrorLog ${APACHE_LOG_DIR}/error.log
     LogLevel warn
     CustomLog ${APACHE_LOG_DIR}/access.log combined
</VirtualHost>

create your flaskapp.wsgi file at your preferred location:

nano flaskapp.wsgi

#!/usr/bin/python
import sys,os
import logging
#Path to your virtualenv
activate_this = 'PATH_TO_VIRTUAL_ENV/activate_this.py'
with open(activate_this) as file_:
    exec(file_.read(), dict(__file__=activate_this))
logging.basicConfig(stream=sys.stderr)
#Location where your server.py file is present.
sys.path.insert(0,"PATH_TO_SRC_DIRECTORY")

from server import app as application

Enable site:

sudo a2ensite flaskapp

Add the site to https:

For encrypting a web connection we need certificate from CA (certificate authority) or we can use self signed certificates. Let's create a self signed certificate using the following command.

openssl req -x509 -newkey rsa:2048 -keyout mykey.key -out mycert.pem -days 365 -nodes
sudo cp mycert.pem /etc/ssl/certs
sudo cp mykey.key /etc/ssl/private

Enable ssl on the server:

sudo a2enmod ssl

Once we create key, we have to add our site and the key to the /etc/apache2/sites-available/default-ssl.conf

sudo nano /etc/apache2/sites-available/default-ssl.conf

Add directory access and path to your website :

# Give an alias to to start your website url with
# Using cmd2web, it means in URL you have to write localhost to access the application. The path after that is path to your wsgi file. Change it accordingly.
WSGIScriptAlias / PATH_TO_WSGI/flaskapp.wsgi
# Mention the directory which should be accessible by your application. This will be directory which has all the files.
            <Directory /home/rohit/Documents/Academics/sem4/IndependentStudy/forkedrepo/cmd2web/>
            # set permissions as per apache2.conf file
                    Options FollowSymLinks
                    AllowOverride None
                    Require all granted
            </Directory>

Locate SSLCertificateFile & SSLCertificateKeyFile and replace the path with the key and certificate file:

SSLCertificateFile  /etc/ssl/certs/cmd2web.pem
SSLCertificateKeyFile /etc/ssl/private/cmd2web.key

Enable the SSL site:

sudo a2ensite default-ssl.conf

Restart the apache server:

sudo service apache2 restart

To access site:

HTTP:
http://localhost/?service=rmsk&chromosome=chr1&start=10000

HTTPS:
https://localhost/?service=rmsk&chromosome=chr1&start=10000

Server address:
http://localhost/

Setting Server:

Currently if there is apache_conf.yaml file, then apache server will be the default server. If on some system that file is not present, then a normal python server can be used.

DB Setup

Tables

1. Groups - It has a list of all the groups with the following fields:
      Name            Type     Primary Key     Description
    - GroupID         Integer  Yes             Group ID of the group
    - GroupName       Text     No              Group Name of the group
    - GroupType       Text     No              Type of the group
    - RestrictedGroup Integer  No              Boolean indicating if group is restricted (1 means restricted)

2. Keys - It has a list of all the keys belonging to a particular user associated to a group.
      Name            Type     Foreign Key     Description
    - GroupID         Integer  Yes             Group ID of the group
    - Token           Text     No              Token for the user
    - Expiry          Text     No              Expiry date of token. Format mm-dd-yyyy
    - UserEmail       Text     No              Email ID of the user

Every Service has a group associated to it. It can either have a value or can be blank. A group if mentioned is a restricted group. If a user calls a service which is restricted, the user needs to pass a token associated with that group provided by the admin. The results are displayed on passing the correct token otherwise an error is displayed.

Command Line Database application for the Admin

The admin has following methods available (DBCommandLineTool.py):

To get a list of all the commands available:

Go to the directory having file DBCommandLineTool.py
Run the following command: 
python DBCommandLineTool.py --help

Output- 
Usage: DBCommandLineTool.py [OPTIONS] COMMAND [ARGS]...

  Command line interface for database interaction.

Options:
  --help  Show this message and exit.

Commands:
  creategroup           Create new group.
  createkeybygroupid    Create new token by group id.
  createkeybygroupname  Create new token by group name.
  deletegroup           Delete group by name.
  deletekeybygroup      Delete key by group name.
  deletekeybyuser       Delete key by group user.
  getkeybygroupname     Get keys by Group.
  getkeybyuser          Get Keys by User.

Click and pandas python library is used to create the command line application and to display the results. All the commands require a user to pass the input parameters. A user can use the help command to find all input parameters required:

1. CreateGroup Command

Input- python DBCommandLineTool.py creategroup --help

Output-
Usage: DBCommandLineTool.py creategroup [OPTIONS]
For eg: python DBCommandLineTool.py creategroup --gname=DummyGroup --gtype=Test --restricted=1

  Description:Create a new group.

  Input parameters required:

  - gname - Group Name

  - gytpe - Group Type

  - restricted - Boolean indicating whether group is restricted

Options:
  --gname TEXT       The group name.
  --gtype TEXT       Type of the group.
  --restricted TEXT  If the group is restricted group. 1 if group is
                     restricted ,0 if not restricted.
  --help             Show this message and exit.



2. CreateKeyByGroupID Command

Input- python DBCommandLineTool.py createkeybygroupid --help

Output-
Usage: DBCommandLineTool.py createkeybygroupid [OPTIONS]
For eg: python DBCommandLineTool.py createkeybygroupid --gid=9 --token=122344434 --expiry=04-27-2019 --email=ro@colorado.edu

  Description:Create  new token by group id.

   Input parameters required:

  - gid - Group ID

  - token - Token for the user

  - expiry - Token expiry date

  - email - Email id of the user

Options:
  --gid TEXT     The group id.
  --token TEXT   Token for the user associated to a group.Format (mm-dd-yyyy).
  --expiry TEXT  Expiry date for the token.
  --email TEXT   Email id of the user.
  --help         Show this message and exit.

Note: The expiry and token command is optional. If not passed random token will be generated and a new date with expiry after a year will be generated.

3. CreateKeyByGroupName Command

Input-python DBCommandLineTool.py createkeybygroupname --help

Output-
Usage: DBCommandLineTool.py createkeybygroupname [OPTIONS]
Foe eg: python DBCommandLineTool.py createkeybygroupname --gname=DummyGroup --token=122344435 --expiry=05-27-2019 --email=rom@colorado.edu

  Description:Create  new token by group name.

   Input parameters required:

  - gname - Group Name

  - token - Token for the user

  - expiry - Token expiry date

  - email - Email id of the user

Options:
  --gname TEXT   The group name.
  --token TEXT   Token for the user associated to a group.
  --expiry TEXT  Expiry date for the token. Format (mm-dd-yyyy).
  --email TEXT   Email id of the user.
  --help         Show this message and exit.

Note: The expiry and token command is optional. If not passed random token will be generated and a new date with expiry after a year will be generated.

4. DeleteGroup Command

Input-python DBCommandLineTool.py deletegroup --help

Output-
Usage: DBCommandLineTool.py deletegroup [OPTIONS]
For eg: python DBCommandLineTool.py deletegroup  --gname=DummyGroup

  Description:Delete group by name.

   Input parameters required:

  - gname - Group Name

Options:
  --gname TEXT  The group name.
  --help        Show this message and exit.


5. DeleteKeyByGroup Command

Input- python DBCommandLineTool.py deletekeybygroup --help

Output-
Usage: DBCommandLineTool.py deletekeybygroup [OPTIONS]
For eg:python DBCommandLineTool.py deletekeybygroup  --gname=DummyGroup

  Description:Delete key by group name.

  Input parameters required:

  - gname - Group Name

Options:
  --gname TEXT  The group name.
  --help        Show this message and exit.


6. DeleteKeyByUser Command

Input-python DBCommandLineTool.py deletekeybyuser --help

Output-
Usage: DBCommandLineTool.py deletekeybyuser [OPTIONS]
For eg: python DBCommandLineTool.py deletekeybyuser --email=rom@colorado.edu

  Description:Delete key by group user.

  Input parameters required:

  - email - email id of the user

Options:
  --email TEXT  The user email.
  --help        Show this message and exit.


7. GetKeyByGroupName Command

Input- python DBCommandLineTool.py getkeybygroupname --help

Output-
Usage: DBCommandLineTool.py getkeybygroupname [OPTIONS]
For eg: python DBCommandLineTool.py getkeybygroupname --gname=DummyGroup

  Description:Get keys by Group.

  Input parameters required:

  - gname - Group name

Options:
  --gname TEXT  The Group name.
  --help        Show this message and exit.

8. GetKeyByUser Command

Input-python DBCommandLineTool.py getkeybyuser --help

Output-
Usage: DBCommandLineTool.py getkeybyuser [OPTIONS]
For eg:python DBCommandLineTool.py getkeybyuser --email=rom2@colorado.edu
  
  Description:Get Keys by User.

  Input parameters required:

  - email - email id of the user

Options:
  --email TEXT  The user email.
  --help        Show this message and exit.

9. GetGroupList Command

Input- python DBCommandLineTool.py getgrouplist --help

Output-
Usage: DBCommandLineTool.py getgrouplist [OPTIONS]
For eg:python DBCommandLineTool.py getgrouplist

  Description:Get all the groups.

  Input parameters required:

  None

Options:
  --help  Show this message and exit.

10. GetKeyList Command

Input- python DBCommandLineTool.py getgrouplist --help

Output-
Usage: DBCommandLineTool.py getkeylist [OPTIONS]
For eg:python DBCommandLineTool.py getkeylist

  Description:Get all the keys.

  Input parameters required:

  None


Options:
  --help  Show this message and exit.

File Upload Service

We need to add the service to the config file. There is a separate endpoint created for the fileupload with "/f" endpoint. File fixed attribute is set to false for this service. For eg:

name : simpleFileGrep
        arguments :
            -
                name : file
                fixed : 'false'
                type : string
            -
                name : pattern
                fixed : 'false'
                type : string
        command :
            - /bin/grep
            - $pattern
            - $file
        output :
            type : text_stream
            sep : \t

The file will be uploaded to the tmp folder and will be deleted once processing is complete. I have implemented it in 3 ways:

1. Curl Command

For eg: If a user wants to call simpleFileGrep service and wants to upload test1.txt file.

Apache curl -X POST -F file=@"test1.txt" "http://localhost/f?service=simpleFileGrep&pattern=zip"

Python curl -X POST -F file=@"test1.txt" "http://127.0.0.1:8080/f?service=simpleFileGrep&pattern=zip"

2. File Upload Command Line Application

The admin has following methods available (DBCommandLineTool.py):

To get a list of all the commands available:

Go to the directory having file UploadFileTool.py
Run the following command: 
python UploadFileTool.py --help

Usage: UploadFileTool.py [OPTIONS] COMMAND [ARGS]...

  Command line interface for database interaction.

Options:
  --help  Show this message and exit.

Commands:
  uploadruncommand  Description: Pass your own input file to a service.

A user can use the help command to find all input parameters required:

1. uploadruncommand Command

Input- python UploadFileTool.py uploadruncommand --help

Output-
Usage: UploadFileTool.py uploadruncommand [OPTIONS]

  Description: Pass your own input file to a service.

   Input parameters required:

  - url - URL with the parameters

  - filepath - Path of the input file

  Example Usage: python UploadFileTool.py uploadRunCommand
  --url='http://127.0.0.1:8080/f?service=simpleFileGrep&pattern=zip'
  --filepath=/home/rohit/test3.txt

Options:
  --url TEXT       The url with parameters
  --filepath TEXT  Path of the input file.
  --help           Show this message and exit.

3. Web Version

Apache https://localhost/webapp

Python http://localhost:8080/webapp

If the file fixed is set to false for the service selected, then the file upload box will appear and user can upload any file from the system.

Name		Name	Last commit message	Last commit date
Latest commit History 49 Commits
DBOperations		DBOperations
DBScript		DBScript
docs		docs
ex_configs		ex_configs
src		src
test		test
web_client		web_client
.gitignore		.gitignore
LICENSE		LICENSE
README.md		README.md
flaskapp.wsgi		flaskapp.wsgi
requirements.txt		requirements.txt

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arguments

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output

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Setting Server:

DB Setup

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1. Curl Command

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1. Curl Command

2. File Upload Command Line Application

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