Bioprinting Strategies

We’re happy to share our latest research on cultured meat, just published in Food Hydrocolloids: Embedded bioprinting enables precise fabrication of cultured meat with authentic structural properties Link: https://lnkd.in/eyJkjNTH In this study, we developed a hybrid fabrication approach combining casting and embedded bioprinting to recreate the complex architecture of natural meat, including detailed marbling structures. By capturing the fine structure of a pork steak via micro-CT and replicating it with embedded bioprinting, we produced cultured meat constructs that closely mimic real meat cuts. The construct includes fibroblasts for the muscle tissue and porcine adipocytes to replicate fat, enabling a closer resemblance to real meat. Below you can find images comparing natural pork and our bioprinted cultured meat — highlighting the potential of this method to bridge the gap between unstructured minced products and structured meat analogues. This work could help improve consumer acceptance of cultured meat by replicating authentic texture and appearance. Thanks to everyone involved in this collaborative effort!

Announcing our latest publication from the #Heilshorn_Biomaterial_Lab! In our new collaborative work, led by brilliant Betty Cai and supervised by Sarah Heilshorn and Sungchul Shin, we developed an integrated fabrication and #endothelialization strategy that directly generates branched, endothelial cell-lined networks using a #diffusion_based, embedded 3D #bioprinting process for the first time. This #innovation not only addresses long-standing challenges in #vascular biofabrication, such as cell uniformity, seeding efficiency, and multi-cell type #patterning but also paves the way for engineering more complex, multi-cellular vasculature. Learn more about how we patterned both #arterial and #venous endothelial cells within a single network to enhance geometric complexity and #phenotypic heterogeneity by reading the full article via the link below: https://lnkd.in/gdcv-hW3 Betty Cai, David Kilian, Julien Roth, Alexis Seymour, Lucia Brunel, Daniel Ramos, @Ricardo J Rios, @Isabella M Szabo, Sean Chryz Iranzo, @Andy Perez, Ram Rao MD PhD, Sungchul Shin, Sarah Heilshorn Stanford University, DTU Health Tech, University of Washington, Seoul National University #Biofabrication #3DBioprinting #TissueEngineering #Bioprinting #VascularEngineering #Endothelialization #Biomaterials #RegenerativeMedicine #BiomedicalEngineering #Innovation #ScientificResearch #CellBiology #VascularNetworks #AdvancedManufacturing #MedicalInnovation #DiffusionBased #EmbeddedBioprinting #MultiCellularSystems #MaterialsEngineering #FutureOfMedicine #Arterial #Venous #ScienceInnovation #HealthcareInnovation #BiomedicalResearch #ScientificPublication

Bridging biomanufacturing and imaging science to engineer the future of regenerative medicine. In our latest publication in Chemical Engineering Journal (CEJ), we present a novel integration of multiple 3D bioprinting modalities with photon-counting computed tomography (PCCT), a next-generation imaging technology offering spectral contrast and ultra-high spatial resolution. Critically, PCCT enables noninvasive, quantitative, and longitudinal imaging of bioprinted implants in vitro and in vivo. This work was made possible through an outstanding collaboration with Dr. Cristian Badea at Duke, whose deep expertise in photon-counting CT was instrumental in developing a robust and translational imaging-engineering pipeline. We see this as a step toward a more tightly integrated ecosystem of biofabrication and imaging, where scaffold design, validation, and optimization can occur in a closed-loop, data-rich, and biologically relevant context. #PhotonCountingCT #3DBioprinting #InVivoImaging #TissueEngineering #RegenerativeMedicine #Biomanufacturing #BiomedicalImaging #HydrogelScaffolds #NoninvasiveImaging #Emory #Duke #GeorgiaTech

I am very happy to share that our most recent paper titled "Advanced bioprinting strategies for fabrication of biomimetic tissues and organs" published by the International Journal of Extreme Manufacturing is available online (https://lnkd.in/dZfHBuWf). This paper discusses the challenges and design requirements in the fabrication of 3D biomimetic tissue constructs, emphasising the need for advanced bioprinting strategies. The focus is on achieving biomimicry, including 3D anatomically relevant structures, biomimetic microenvironments, and vascularisation. Various advanced bioprinting strategies are discussed in detail, including advancements in both fabrication techniques and bio-inks. Future directions in advanced bioprinting systems are outlined, with special attention to multi-modal bioprinting systems, in-situ bioprinting, and the integration of machine learning into bioprinting processes. The critical role of bio-inks and printing methodologies in influencing cell viability is highlighted, providing insights into strategies for enhancing cellular functionality throughout the bioprinting process. The paper also addresses considerations post-fabrication, particularly in accelerating tissue maturation, as a pivotal component for advancing the clinical applicability of bioprinted tissues. The paper navigates through the challenges, innovations, and prospects of advanced bioprinting strategies, highlighting their transformative impact on tissue engineering. Thank you to all co-authors Ng Wei Long, Cian Vyas, BOYANG HUANG, Wai Yee Yeong 👏 ➡️ I hope you enjoy reading the paper! #3dbioprinting; #insituprinting; #bioinks; #biomimicry; #vascularisation; #cells; #tissueengineering

4D bioprinting of self-forming tubular structures utilizing functionally modified silk methacrylate (SilMA) Read more: https://lnkd.in/gzMs8Tvh The recent research out of LUBNA Z. joint Deakin-IITH #PhD presents a novel #4Dbioprinting strategy for fabricating vascularised #tubular structures using #silk cocoon extract (SilMA) and its composites. By exploiting nature-inspired morphing mechanisms, the study demonstrates extrusion-based printing with visible-light crosslinking to create self-supporting hollow tubes (700–1000 μm) that undergo rapid structural transformations within one minute of aqueous stimulation. Systematic optimisation of aspect ratio, crosslinking time, and material concentrations enabled high precision in tailoring #morphing behaviour, #swelling response, tensile strength, and #biocompatibility for supporting cell growth. Importantly, the work highlights that not all SilMA–CMC compositions are suitable, proposing chemical tailoring to achieve viscosity control without compromising 4D functionality. This breakthrough establishes functionally modified silk as a #dynamic biomaterial platform for #vascular #tissue #engineering and broader biomedical applications, setting a benchmark for adaptable, high-performance constructs in regenerative medicine and materials science. Authors: LUBNA Z., Ali Zolfagharian, Ashis Kumar Bera, Jaideep Adhikari, Falguni Pati #deakinuniversity #deakin Deakin University Indian Institute of Technology Hyderabad #4dprinting #3dprinting #smartmaterials #additivemanufacturing

🫀 A Heart Printed From Life — The Future of Medicine Is Already Here In a breakthrough that pushes regenerative medicine into a new era, scientists have successfully 3D-printed a human heart made entirely from a patient’s own living cells — a feat once considered pure science fiction. Using cutting-edge bioprinting technology, researchers begin by converting a small tissue sample into personalized bio-ink, rich with cells capable of forming cardiac muscle, blood vessels, and structural proteins. Layer by layer, the printer constructs a fully shaped heart — complete with chambers, valves, and intricate vascular networks engineered to function like the real organ. Unlike mechanical implants or donor organs, this bioprinted heart carries the patient’s exact cellular identity, dramatically reducing the risk of rejection. Scientists explain that the printing process mimics natural embryonic development, guiding cells to self-organize into beating tissue as electrical impulses begin to pulse through the structure. Early prototypes have already shown rhythmic contractions in the lab, proving that these aren’t just anatomical models — they’re alive. The implications for global healthcare are immense. With donor shortages affecting millions, a future where patients receive personalized organs printed on demand could redefine transplant surgery. Conditions once deemed fatal may one day be treated with organs grown from the patient’s own cells — improving survival rates and eliminating lifelong immunosuppressant therapy. Though clinical implantation in humans is still under development, experts agree: This achievement marks one of the most profound steps toward custom-made, living human organs — printed with precision, powered by biology, and built from the patient themselves. #Medical #Biotech #Regeneration #fblifestyle #Technologia --- 📚 Reference Section (Selected Scientific Sources) 1. Tal Dvir et al. (2019) – “3D Printing of Personalized Thick and Fully Vascularized Heart Tissues.” Advanced Science. 2. Murphy, S. V. & Atala, A. (2014) – “3D bioprinting of tissues and organs.” Nature Biotechnology. 3. Lee, A. et al. (2019) – “Three-dimensional bioprinting of functional human tissues.” Nature Protocols. 4. Noor, N. et al. (2019) – “3D Printed Cardiac Patches and Hearts from Patient Cells.” Advanced Science. 5. Vega, S., Kwon, M. et al. (2023) – “Engineering functional cardiac tissues through bioprinting.” Biomaterials. #ashishdrishti👀📚🧬 #everyone