Addgene

Nothing but next-generation sequencing drives this approach to minimizing off-target CRISPR cleavage. The concept is simple: by choosing the least active Cas9 variant that still efficiently cuts your target site, you can reliably minimize off-target cleavage. Check out our post by guest blogger Balázs Csoma to learn how to select the right Cas9 from the Welker Lab's 17-variant CRISPRecise kit in only two steps. https://hubs.ly/Q048_TTM0

Every plasmid in Addgene's collection represents someone's hard work. When a researcher shares that work with the world, the community should be able to access that exciting new tool, as a fully verified construct, without delay. Delivering on that means continuously improving how we efficiently and accurately sequence what comes into our ever-growing collection. Our new partnership with Plasmidsaurus, a leading Sequencing as a Service company, is how we scale that commitment. Plasmidsaurus earned this partnership for good reason — they've sequenced over 5 million plasmids since 2021, delivering whole-plasmid results overnight with consensus accuracy that frequently reaches Q60 (one error per million bases). A single pass covers the entire plasmid, making it particularly reliable for constructs with complex or repeated features. If you haven't tried their platform yet, now's a great time. They're offering the Addgene community their first three sequences free with code ADDGENE. https://hubs.la/Q0485h120

When values align this well, you just say yes. Quality, efficiency, open science — and an appreciation for a well-drawn T-Rex. 🎨🦕 Here's to open science, fast sequencing, and more dinosaurs.

Whatever your CNS gene transfer experiment calls for — Addgene has an AAV for that. With 1,000+ in-stock preparations and 30,000+ on-demand options, the collection keeps growing with new deposits every day. Browse AAV preparations: https://hubs.la/Q0482r6f0

Introducing Saved Lists on Addgene! Never lose track of a reagent again. As you browse plasmids, viral vectors, pooled libraries, antibodies, and more, you can now save items, organize them into custom lists, and share them with collaborators whenever you're ready. Whether you're planning experiments, building a reagent wishlist, or coordinating with your lab, Saved Lists make it easier to stay organized and keep your team on the same page. Try Saved Lists today: https://hubs.la/Q0482qM-0

Every day, researchers around the world are developing and sharing exciting new tools. Making sure those materials are verified and available to the scientific community as quickly as possible is at the heart of what we do. Today we're excited to announce our new partnership with Plasmidsaurus — a four-year commitment to scale how we sequence and verify what comes into our ever-growing collection. Plasmidsaurus, a leading Sequencing as a Service company, will handle the majority of Addgene's whole-plasmid sequencing using long-read sequencing technology. Addgene will continue to perform the rigorous QC and analysis our community knows and trusts. Read the full announcement: https://hubs.la/Q0482vsY0

This is exactly why we created the Blue Flame Award! To celebrate labs that make open science real. So proud to recognize this team for their commitment to sharing their work with the community.

We love seeing the CellCousin plasmids making their way to labs around the world! Thank you for depositing these tools and making them accessible to the research community — open reagent sharing like this is what accelerates science. And nice T-shirt!

Choosing the right AAV serotype is an important step in designing successful in vivo experiments — since tropism can vary widely across tissues and applications. Addgene’s AAV Packaged on Request service offers multiple serotypes, including AAV9, giving researchers more flexibility for CNS, cardiac, and muscle delivery work. With Packaged on Request, you can select your preferred cargo plasmid, serotype, and volume, and receive QC’d viral prep backed by the same stringent standards as our in-stock preparations. Interested in how different serotypes perform across tissues? Read more in our blog: https://hubs.la/Q042S6cy0

Addgene was pleased to host Steve Owens, Katherine Tolley from Representative Owens’ office, Griffin Tighe from Speaker Pro Tempore Kate Hogan’s office, and Anne Mazzola from Senator Will Brownsberger's office last week. During their visit, we had the opportunity to introduce Addgene’s mission, highlight our role in advancing scientific research through material sharing, and offer a look inside our laboratory operations. We are thankful for their visit and for their interest in the work being done across Massachusetts to strengthen the life sciences ecosystem and support innovation.