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Artikel von Andre Heeg, MD
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Nobody needs a £90 candle. That’s exactly why branding matters. Because logically, a £2 IKEA candle and a £90 Diptyque candle do the same thing...…
Nobody needs a £90 candle. That’s exactly why branding matters. Because logically, a £2 IKEA candle and a £90 Diptyque candle do the same thing...…
Beliebt bei Andre Heeg, MD
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For over 40 years, positioning has only been defined by a single dimension - differentiation. Nothing has really changed since the original book by…
For over 40 years, positioning has only been defined by a single dimension - differentiation. Nothing has really changed since the original book by…
Beliebt bei Andre Heeg, MD
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Executive retention is not about a bigger bonus. So many companies are getting this completely wrong... And it’s costing them excellent executive…
Executive retention is not about a bigger bonus. So many companies are getting this completely wrong... And it’s costing them excellent executive…
Beliebt bei Andre Heeg, MD
Berufserfahrung und Ausbildung
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The Upward ARC
Gesamte Berufserfahrung von Andre Heeg, MD anzeigen
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Bescheinigungen und Zertifikate
Veröffentlichungen
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3D-Bioplotting with vitale cells
Doctor thesis
Veröffentlichung anzeigenPurpose of this thesis was to test a concept of 3D-bioplotting with vital KB cells. This concept included a freezing process with liquid nitrogen and allowed composing three-dimensional alginate scaffolds with vital cells integrated in the single strings of the scaffold.
Therefore firstly vital KB cells stemming from a permanent cell line of a mucoepidermiod carcinoma where integrated in an alginate suspension. We evaluated various compositions of alginate and calcium chloride solutions as…Purpose of this thesis was to test a concept of 3D-bioplotting with vital KB cells. This concept included a freezing process with liquid nitrogen and allowed composing three-dimensional alginate scaffolds with vital cells integrated in the single strings of the scaffold.
Therefore firstly vital KB cells stemming from a permanent cell line of a mucoepidermiod carcinoma where integrated in an alginate suspension. We evaluated various compositions of alginate and calcium chloride solutions as well as parameters of the plotting process, especially the cooling process with liquid nitrogen. The solutions and suspensions were tested with an osmometer regarding their osmotic pressure. After fabricating the three-dimensional grid constructs with the 3D-Bioplotter we cultivated the scaffolds for one week. After that cultivation time we investigated vitality and proliferation of the cells using PI/FDA fluorescence microscopy and EZ4U proliferation tests.
The results showed that surviving of in alginate integrated and directly plotted KB cells in three-dimensional scaffolds using a cooling system with liquid nitrogen is possible. A relevant part of the integrated KB cells was still vital after the full week of cultivation.
Alginate is very applicable for usage as biomaterial for fabrication of three-dimensional cell containing scaffolds. Fixation of plotted 3D grid constructs via freezing and following thawing via calcium chloride solution is a concept that allows protection of vitality of integrated living cells for several days. Further development of this concept by optimizing compositions of alginate solutions and by usage of suitable anti-freezing agents during the freezing process will be subject of further investigations. -
The Immunobiology of allograft rejection: Characterisation of antigen-specific MHC class II positive CD4+ T cells
Doctor thesis
Veröffentlichung anzeigenThe phenomenon of T cell stimulation by MHC class II expressing (MHC IIpos) CD4+ T cells has been intensively investigated for T cell clones but, so far, not for native T cells. The extensive use of T cell clones may explain the inconsistent outcomes of T cell-mediated antigen-presentation. Therefore, we used freshly isolated primed rat CD4+ T cells induced by immunisation with an allogeneic peptide P1, which is involved in allograft rejection.